Ascorbic acid determination

Cyclic voltammetry and spectrophotometry showed good agreement for the antioxidant capacity estimation in buckwheat products after hydrothermal treatment [ 35 ]. This will eventually affect the results of the experiments. Using a 25 ml transfer pipette, transfer 25ml of the vitamin C solution into a ml Erlenmeyer conical flask.

This is because it might affect the rate of the reaction or produce an inaccurate reading which can affect the results. In the cells it is easily oxidized to dehydroascorbic acid, removing oxidizing agents before they can do damage to other substances present. Many analytical methods can be used for ascorbic acid determination.

Therefore, the rate of reaction may differ from each other and hence the accuracy might reduce. The animal species, which are able to produce this molecule, biosynthesise AA from glucose catalyzed Lgulonolactonoxidase [ 12 Ascorbic acid determination.

Iodometric titration of vitamin C (ascorbic acid)

However, Ascorbic acid determination evidence does not support a role for high-dose vitamin C in the treatment of cancer or the prevention of the common cold in the general population. Fill up to mL in the volumetric flask. The developed method was applied to the determination of ascorbic acid in different fruit juice, and the obtained results were compared with those obtained by a conventional titrimetric method.

This assay was finally conducted at the University of Pittsburgh in the laboratory of King, which had been working on the problem for years, using guinea pigs. The results obtained in this study show that cyclic voltammetry can be successfully used as part of quality management in food industry, for assessing the vitamin C content in natural fruit juice and soft drinks.

Recently, the use of various voltammetric techniques has been combined with modified ascorbic acid sensors; square-wave voltammetry was used to determine ascorbic acid based on its oxidation at a zeolite modified carbon paste electrode [ 32 ], and the method was applied to ascorbic acid determination in citrus juice.

These physicians were investigating dietary-deficiency diseases using the new animal model of guinea pigswhich are susceptible to scurvy. Add 5 mL of starch solution. Iodine solutions are not Ascorbic acid determination and they should be standardized every months.

Different ascorbic acid concentrations from standard solutions were added to the analysed samples, the degree of recovery being comprised between Thus, the reported values for lemon are Published online Nov 7. Voltammetry is an increasingly popular method applied to the determination of ascorbic acid in real samples [ 7 ], because it offers low detection limits, even when compared to more expensive techniques.

Ascorbic acid determination results obtained by cyclic voltammetry were compared with those obtained by the volumetric method with dichlorophenol indophenol.

The newly discovered food-factor was eventually called vitamin C. This sensor was constructed by aniline electropolymerization on a glassy carbon or a screen-printed working electrode.

Calculate the mass mg of ascorbic acid in the vitamin C tablet and thus the mass percentage of ascorbic acid. In the cells it is easily oxidized to dehydroascorbic acid, removing oxidizing agents before they can do damage to other substances present.

The details about ascorbic acid antioxidant system cooperated with glutathione was described by Meister [ 6 ]. The ascorbic acid content determined ranged from 0.

View at Google Scholar P.One way to determine the amount of vitamin C in food is to use a redox titration. The redox reaction is better than an acid-base titration since there are additional acids in a juice, but few of them interfere with the oxidation of ascorbic acid by iodine.

solution of ascorbic acid with NaOH to the faint pink phenolphthalein end point: HC 6 H 7 O 6 + NaOH > C 6 H 7 O 6-+ H 2 O + Na + (2) ascorbic acid ascorbic ion Equation (2) is a reaction, where one mole of NaOH added reacts with one mole of ascorbic acid present in the solution.

solution of ascorbic acid with NaOH to the faint pink phenolphthalein end point: HC 6 H 7 O 6 + NaOH > C 6 H 7 O 6-+ H 2 O + Na + (2) ascorbic acid ascorbic ion Equation (2) is a reaction, where one mole of NaOH added reacts with one mole of ascorbic acid present in the solution.

This paper describes an experimental determination of ascorbic acid using a sample which is easily prepared and analyzed, and sufficiently stable to be suitable for use as an unknown in the quantitatively oxidized by iodine in acid solution. A new, simple and sensitive method for the spectrophotometric indirect determination of ascorbic acid in fruits, beverages, and pharmaceuticals is described.

Journal of Analytical Methods in Chemistry

In this method, the ascorbic acid reduces Cu 2+ to Cu + and reacts. Ascorbic acid and its sodium, potassium, and calcium salts are commonly used as antioxidant food additives. These compounds are water-soluble and, thus, cannot protect fats from oxidation: For this purpose, the fat-soluble esters of ascorbic acid with long-chain fatty acids (ascorbyl palmitate or ascorbyl stearate) can be used as food antioxidants.

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Ascorbic acid determination
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